Clear Colourful Acid Fast Stain Reagents Phenol Free Fuchsin Staining Method For Auto Stainer

Acid Fast Stain (Phenol-free fuchsin staining method for auto stainer) Baso Clear Colourful Stains

Testing Principle

Acid-fast bacteria, such as Mycobacterium (e.g., Mycobacterium tuberculosis), have a lipid or wax-like membrane on their surface, making them difficult to stain. However, once stained, they resist decolorization even when treated with acid-alcohol. This characteristic is utilized by first staining with an enhanced staining solution, then treating with acid-alcohol to decolorize non-acid-fast bacteria, followed by a counterstain. As a result, acid-fast bacteria retain the initial stain color (red), making them easy to identify.

This product improves upon the traditional Ziehl-Neelsen acid-fast staining method by modifying the carbol fuchsin solution to exclude phenol (also known as carbolic acid) while still allowing acid-fast bacteria to stain a vivid red. The counterstain uses methylene blue, creating a sharp contrast between the color of the acid-fast bacteria (red) and the background (blue).

Stain Results
Against a light blue background, acid-fast bacteria (e.g., Mycobacterium tuberculosis) appear red, while other bacteria and cells appear blue.

Acid-fast bacteria (using Mycobacterium tuberculosis as an example):Most are rod-shaped and slightly curved.The width of the bacteria ranges from 0.3~0.6 μm.The length varies between 0.5~8 μm, with most falling within 1.5~3.5 μm.A few longer bacteria may appear spiral-shaped.In well-stained Mycobacterium tuberculosis, deeply stained metachromatic granules can be observed within the bacterial cells.In fresh specimens containing a high concentration of Mycobacterium tuberculosis, after acid-fast staining and observation under a 100× oil immersion lens, individual bacteria can be seen, as well as clusters or branching arrangements of bacteria.

Packaging Specification

acid fast stain reagents Phenol Free

Clear Colourful acid fast stain reagents

reagents used for acid fast staining

Clear Colourful Acid Fast Stain Reagents Phenol Free Fuchsin Staining Method For Auto Stainer Images